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Mice really should receive sulfamethoxazole (two hundred mg sulfamethoxazole furthermore 40 trimethoprim in 225 mL h2o; Schein Pharmaceutical, Florham, NJ) in their consuming drinking water for one wk right after surgical procedure, to reduce the potential risk of an infection. The mice are observed everyday for the main 7 days right after surgical procedures, to guarantee which the animals are eating, drinking, and behaving Typically and that they've got not removed the bandage or ruined the graft site. When the bandage continues to be taken out, the dressing pad and self-adhering bandage are reapplied.
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Unaffected controls are made through the use of age- and sexual intercourse-matched C3H/HeJ mice without any scientific proof of AA. Skin is prepared as explained before, as well as the surgery is done in the same way.
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